客服热线:400-901-9800          客服QQ:4009019800          技术答疑     技术支持     质量反馈     联系我们
首页 > AMG88 > 一抗 > 产品信息
Rabbit Anti-HMGB1 antibody (bs-0664R)
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:support@bioss.com.cn
说明书:50ul  100ul  200ul
20ul/380.00元
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号bs-0664R
英文名称HMGB1
中文名称高迁移率族蛋白B1抗体
别    名Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; High mobility group protein B1; HMG3; HMGB 1;HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.  
Specific References  (7)     |     bs-0664R has been referenced in 7 publications.
[IF=3.24] Zhao, Lu, et al. "The high mobility group box 1 protein of< i> Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation." Developmental & Comparative Immunology 35.10 (2011): 1052-1058.  WB ;  
[IF=3.12] Jiang, W., et al. "Curculigoside A attenuates experimental cerebral ischemia injury< i> in vitro and< i> vivo." Neuroscience 192 (2011): 572-579.  WB ;  Rat.  
[IF=3.05] Rui, Zhang, et al. "Omega-3 polyunsaturated fatty acids inhibit the increase in cytokines and chemotactic factors induced in vitro by lymph fluid from an intestinal ischemia-reperfusion injury model." Nutrition (2014).  WB ;  Rat.  
[IF=0.94] Wang, Xin‑Jun, et al. "Clinical and prognostic significance of high-mobility group box-1 in human gliomas." Experimental and Therapeutic Medicine.  WB ;  Human.  
[IF=1.34] Sun, Shanping, et al. "High mobility group box-1 and its clinical value in breast cancer." OncoTargets and Therapy 8 (2015): 413.  IHC-P ;  Human.  
[IF=2.61] Li, Yuan-bo, et al. "Methotrexate affects HMGB1 expression in rheumatoid arthritis, and the downregulation of HMGB1 prevents rheumatoid arthritis progression." Molecular and Cellular Biochemistry: 1-10.  Human.  
[IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775.  WB ;  Rat.  
规格价格50ul/780元购买    100ul/1380元购买    200ul/2200元购买    大包装/询价
说 明 书50ul  100ul  200ul
研究领域肿瘤  细胞生物  免疫学  转录调节因子  结合蛋白  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Cow, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量25kDa
细胞定位细胞核 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human HMGB1:75-170/215 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models

Function:
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Subunit:
Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2.

Subcellular Location:
Nucleus. Chromosome.

Similarity:
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.

SWISS:
P09429

Gene ID:
3146

Database links:

Entrez Gene: 282691 Cow

Entrez Gene: 3146 Human

Entrez Gene: 100862258 Mouse

Entrez Gene: 15289 Mouse

Entrez Gene: 25459 Rat

Omim: 163905 Human

SwissProt: P10103 Cow

SwissProt: P09429 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

近来的研究表明称之为高迁移率族蛋白B-1(HMG-B1)的核内结构蛋白在核外表达时是一种有效的早期炎症介质。
高迁移性B1组蛋白(HMGB1): 是一种核结合蛋白,在DNA重组、修复、复制和基因转录中起作用。HMGB1也是巨噬细胞分泌的一种介质。
此外,高迁移性B1组蛋白亦被受刺激的巨噬细胞或单核细胞主动分泌。在这一主动分泌过程中,HMGB1首先经乙酰化并由核内转移至溶酶体内,继而在ATP和溶血磷脂胆碱两种分泌信号指导下转移至细胞外。由坏死细胞被动释放的HMGB1和炎症细胞主动分泌的HMGB1存在分子上的差异。胞外的HMGB1可作为细胞因子参与信号传导,因为它既可识别Toll 样受体(TLR)家族的一些成员,又能与识别晚期糖基化终末产物受体(RAGE)相作用。
HMGB1能启动炎症反应,包括产生多种细胞因子、对某些干细胞产生趋化作用、诱导血管粘附分子、削弱肠上皮细胞的功能等。
产品图片
Sample: bone (mouse) Lysate at 40 ug
Primary: Anti- HMGB1(bs-0664R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27kD
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-0664R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGB1 Polyclonal Antibody, Unconjugated(bs-0664R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGB1 Polyclonal Antibody, Unconjugated(bs-0664R) 1:400, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-0664R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Rat splenocytes stained with Anti- HMGB1 Polyclonal Antibody, A488 Conjugated (bs-0664R-A488) at 1:50.
The figure annotation:
The blue histogram is unstained cells.The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC)The green histogram is cells stained with Rabbit Anti-HMGB1/FITC Conjugated antibody (bs-0664R-FITC).
Controls
Positive control: HepG 2 cellsIsotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC). instead of the primary antibody to confirm that primary antibody binding is specific. 5μgin 100 μL 1 X PBS containing 0.5% BSA.
Positive control: HepG2 cells
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
版权所有 2016-2020 www.bioss.com.cn 亚美游AMG88|mg篮球巨星游戏【正规牌照网址!】 京ICP备05066980号 京公网安备110107000727号
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100